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 Dr. Ashwil Klein

Position: Lecturer
Department: Department of Biotechnology
Faculty: Faculty of Natural Science
Qualifications: BSc, BSc Hons, MSc, PhD (UWC
Tel: 021 959 3327
Fax: 021 959 3505
Email: aklein@uwc.ac.za
Research:
 
 
 
 

Biography

 Dr Klein holds a PhD in Biotechnology from the University of the Western Cape. At the end of 2013, he completed one year of postdoctoral fellowship in Plant Biotech Research Group (PBRG) under the guidance of Prof Ndiko Ludidi at the University of the Western Cape, for which he was awarded the National Research Foundation Research Postdoctoral Fellowship. In 2014, he was appointed in the Department of Biotechnology as Lecturer on a two year contract.
Dr Klein’s research interest focus on elucidating the effect of exogenous application of small plant signaling molecules (cGMP and caffeic acid) in regulating plant responses to drought and salinity stress. This work aims to establish a biotechnology strategy to improve drought and salinity tolerance in leguminous plants by investigating the following:
1) The effect of exogenous applied of signaling molecules on legume antioxidant enzyme activity.
2) The effect of signaling molecules on the expression of genes encoding legume antioxidant enzymes.
3) The effect of signaling molecules on the levels and identity of proteins accumulated in legumes.
4) Identification of differential expressed genes in legume plants in response to treatment with exogenously applied signaling molecules by cDNA-AFLP analysis.

Publications

 Research articles:
  •  Keyster M, Klein A, Ludidi N (2010) Endogenous NO levels regulate nodule functioning: Potential role of cGMP in nodule functioning? Plant Signaling & Behavior, 12, 1-3. 
  •  Keyster M, Klein A, Egbichi I, Jacobs A, Ludidi N (2011) Nitric oxide increases the enzymatic activity of three ascorbate peroxidase isoforms in soybean root nodules. Plant Signaling & Behavior, 6: 1-6. 
  • Keyster M, Klein A, Ludidi N (2012) Caspase-like enzymatic activity and the ascorbate-glutathione cycle participate in salt stress tolerance of maize conferred by exogenously applied nitric oxide. Plant Signaling & Behavior, 7 (3), 349-360. 
  • Keyster M, Klein A, Du Plessis M, Jacobs A, Kappo A, Kocsy G, Galiba G, Ludidi N (2012) Capacity to control oxidative stress-induced caspase-like activity determines the level of tolerance to salt stress in two contrasting maize genotypes. Acta Physiologiae Plantarum, 35 (1), 31-40. 
  • Keyster M, Adams R, Klein A, Ludidi N (2013) Nitric oxide regulates expression of a soybean cystatin and influences caspase-like activity in soybean root nodules. Plant Omics: 6 (3), 183-192. 
  • Klein A, Keyster M, Ludidi N (2013) Caffeic acid decreases salinity-induced root nodule superoxide radical accumulation and limits salinity-induced biomass reduction in soybean. Acta Physiologiae Plantarum, 35 (10), 3059-3066. 
  •  Egbichi I, Keyster M, Jacobs A, Klein A, Ludidi N (2013) Modulation of antioxidant enzyme activities and metabolites ratios by nitric oxide in short-term salt stressed soybean root nodules. South African Journal of Botany, 88, 326-333. 
  •  Klein A, Ludidi N (2014) Nitric oxide restricts salt-induced oxidative damage in maize by altering the activity of various superoxide dismutase isoforms. Acta Physiologiae Plantarum. Research article submitted. 
  • Klein A, Keyster M, Ludidi N (2014) Exogenously applied caffeic acid elevates nitric oxide content and restricts salinity-induced oxidative damage in soybean nodules. South African Journal of Botany, Research article submitted. 
 Conference proceedings:
  • Maria M. van Dyk, John Baison, Paidashe Hove, Claudius Marondedze, Ashwil Klein, Khashief Soeker and D. Jasper G. Rees (2007) Bin-mapping of EST-SSR and EST-SNP in apple (Malus x domestica.). Eucarpia XII Fruit Section Symposium, September 16-20, 2007, Zaragoza, Spain, 57-58. 
  • Marshall Keyster, Alex Jacobs, Ashwil Klein, Morné Du Plessis, Paul Kappo, Gábor Kocsy, Gábor Galiba, Ndiko Ludidi (2010) Two maize genotypes demonstrate the importance of antioxidant capacity and cysteine endopeptidase activity in regulating plant tolerance to salt stress. 4th International Symposium of the SFB 429, Potsdam Germany. 
  • Ashwil Klein, Marshall Keyster, Ndiko Ludidi (2012) Nitric oxide mediated signaling for enhancing maize tolerance to abiotic stress. SASBMB/FASBMB Congress, Champagne Sports Resort, Drakensberg, South Africa, 29 January – 01 February 2012. 
  • Marshall Keyster, Nashiphi Silulwane, Ashwil Klein, Alex Jacobs, Ndiko Ludidi (2012) Nitric Oxide regulates expression of a soybean Cystatin and influences Caspase-like activity in soybean root nodules. SASBMB/FASBMB Congress, Champagne Sports Resort, Drakensberg, South Africa, 29 January – 01 February 2012. 
  • Ashwil Klein, Marshall Keyster, Ndiko Ludidi (2013) Caffeic acid-induced salt stress tolerance is mediated by nitric oxide and hydrogen peroxide scavenging enzymes in soybean root nodules. 39th Annual Conference of the South African Association of Botanists (SAAB), At Drakensberg, South Africa, Volume: 86. 
  • Marshall Keyster, R. Adams, Ashwil Klein, Ndiko Ludidi (2013) Nitric oxide differentially modulates the expression of four cystatins from soybean. 39th Annual Conference of the South African Association of Botanists (SAAB), At Drakensberg, South Africa, Volume: 86. 
  • Ashwil Klein, Ndiko Ludidi (2014) Nitric oxide restricts salt-induced oxidative damage in maize by altering the activity of various superoxide dismutase isoforms. SASBMB Congress, Goudini Spa, Rawsonville, South Africa, 06 July – 09 July 2014.
  • Ndiko Ludidi, Zinthle Kolo, Ashwil Klein, Neo Ramorara (2014) ZmC3H is a candidate Zea mays p-coumarate 3-hydroxylase involved in regulating maize tolerance to drought. SASBMB Congress, Goudini Spa, Rawsonville, South Africa, 06 July – 09 July 2014.
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Networks

 Dr Klein is in the process of expanding his research portfolio under the mentorship of Prof Ndiko Ludidi (UWC) and Prof Bongani Ndimba (UWC-ARC).
Society membership:
• South African Society of Biochemistry and Molecular Biology (SASBMB)
• American Society of Plant Biologists (ASPB)
• South African Association of Botanist (SAAB)
Representative on Departmental/faculty committee:
• Faculty Research Open Day - Biotechnology
• Departmental Webpage liaison officer – Biotechnology
• Biotechsafe – Plant Biotechnology

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