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The Next Generation Sequencing Facility

Author:  Institute for Microbial Biotechnology and Metagenomics

The facility is perfectly suited to conduct amplicons-based projects as well as sequencing of bacterial and viral genomes.

The Next Generation Sequencing Facility (NGSF) was established by Prof Cowan (former director of IMBM), with funding obtained from the NRF’s large equipment grant in 2011. The NGSF houses two high throughput sequencers. The Roche GS Junior Genome Sequencer, is a benchtop version of the larger FLX Genome Sequencer and is based on the same Titanium sequencing technology.

The output of the GS Junior is estimated to be 35 million bp per run for shot gun sequencing and 24 million bp per run for amplicon sequencing. In June 2012, the Facility acquired an Illumina MiSeq. The output of this sequencer is currently 1 Gb, however, with the newly released sequencing technology of the sequencing kits, by December 2012 the outputs of the MiSeq will be increased to 7 Gb.
 

356The NGSF has been established as a national platform to serve the next generation sequencing needs of the local academic community. By offering two complementary sequencing technologies, the facility is perfectly suited to conduct amplicons-based projects (HIV resistance, drug resistance, 16S rRNA gene analysis), as well as sequencing of bacterial and viral genomes, BACS and fosmids and metagenomics.
 
The NGS Facility is housed on the third floor of the new Life Science building. The facility is managed by Dr Bronwyn Kirby and Ms Nondumiso Zondi acts as the Senior Technician. For more information on the NGSF please contact Dr Kirby at bkirby@uwc.ac.za or sequence@uwc.ac.za , office 3.55 3rd level block B, New life science building.

 

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