| Instrumentation | Description | Use/Type of analysis |
| MALDI-TOF-IMS ( UltrafleXtremeTM) |
MALDI-TOF Imaging Mass Spectrometry of down to 5µm animal and plant tissue cross-sections, based on lipid, metabolite, protein and peptide profiles | Spatial distributions of m/z peak intensities |
| MALDI Biotyper ( UltrafleXtremeTM) |
Microbial typing by Linear MALDI-TOF Mass Spectrometry of agar plated cultures, directly spotted or extracted for ribosomal proteins | Pattern matching, scoring of microbial identities, principal component analysis and hierarchical clustering/dendrogram analysis |
| LC-MALDI – MS ( UltrafleXtremeTM) |
EASY Nano-RPLC-II separation, Proteineer fc-II spotting and subsequent MALDI MS and LIFT (MS/MS) analyses for shotgun proteomics, glycomics and metabolomics | Multiple and comprehensive representations of proteomics and other omics data in terms of lists of protein ID’s, database matched peptide lists, MS and MS/MS spectra, associated FASTA sequences, associated PDB 3D structure profiles, PTM’s and gene ontology analysis, etc |
| TDS MALDI analysis ( UltrafleXtremeTM) |
Top-down sequencing of purified intact proteins and polypeptides (MW < 50 kDa) by LIFT MS/MS, followed by automatic deNovo sequencing | Near 100 % amino acid sequence coverage and MW information. |
| TLC-MALDI ( UltrafleXtremeTM) |
2D Imaging by MS and LIFT MS/MS of lipid and metabolite spots directly on thin-layer chromatography (TLC) plates | 2D profiling in terms of TLC, LipidMaps based identification and LIFT analysis of putative lipids. |
| PharosFX Plus Molecular Imager, Spot Cutter (Gel Imaging and Spot Cutting) |
Scan images at resolutions from 50-800 microns. It has a linear dynamic range that extends over 4.8 orders of magnitude. | Having 6 filters which use image dyes Scans multi-color fluorescence applications and phosphotor images |
Company Projects
| Name | Project | Company/Institution | Authorized by: |
| Ms Donna-Leigh van Staden | SDS PAGE Analysis of Collagen Type I samples from The Stellenbosch Nanofiber Company (SNC.) | The Stellenbosch Nanofibers Company | Prof Bongani Ndimba |
| Dr Arnold Kanengoni | Analysis of diet-induced differential proteomics in indigenous pig (IP) and large white cross races (LWR.) | Agricultural Research Council (ARC) - Animal Production Institute | Prof Bongani Ndimba |
| Ms Roya Ndimba | Differential proteomic analysis of prolamin extracts in wild-type sorghum and variant strains. | Materials Research Group, iThemba LABS, National Research Foundation (NRF) |
Prof Bongani Ndimba |
| Gcineka Mbambisa | MALDI-MS Analysis of polyanilines derivative end chains. | Department of Chemistry University of the Western Cape |
Prof Bongani Ndimba |
| MC Pilane | Cancer Proteomics 2D gel study (Limpopo project.) | Department of Biochemistry, Microbiology and Biotechnology University of Limpopo |
Prof Bongani Ndimba |
| Mr Cornelius J. F. Taute | Analysis of biocompatible polymer (PEI - 2000), used in bio-nanosynthesis for drug-delivery and gene transfection. | Nanotechnology Innovation Centre Biolabels Unit Unversity of the Western Cape |
Prof Bongani Ndimba |
| Rufaro Chivaura | An investigation of the development of a biochemical and clinical marker for gastric disease. | Department of Surgery University of Cape Town |
Prof Bongani Ndimba |
| Clifford Ntui | Structural Analysis of Car D protein 1. | Structural Biology Unit Biotechnology Department University of the Western Cape |
Prof Bongani Ndimba |
| Lonnie van Zyl | Biotyper Project. | Institute for Microbial Biotechnology and Metagenomics University of the Western Cape |
Prof Bongani Ndimba |
| Dr Rudo Ngara | Analysis and Sequencing/Identification of 20 protein spots (Comparative leaf Proteomics of different Sorghum varieties under salinity stress. | Proteomics Unit Department of Biotechnology University of the Western Cape |
Prof Bongani Ndimba |
| Dr Rudo Ngara Chipampe Lombe |
Comparative leaf Proteomics of different Sorghum varieties under salinity stress. | Proteomics Unit Department of Biotechnology University of the Western Cape |
Prof Bongani Ndimba |
| Demonstration/Consumables Venue and Facilites |
4th Proteomics Workshop | UWC, ACGT, UCT, UP,UJ, University of Cambridge, CSIR | Prof Bongani Ndimba |
